ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2015, Vol. 46 ›› Issue (6): 932-939.doi: 10.11843/j.issn.0366-6964.2015.06.008

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The Effects of FSH,LH on Apoptosis and E2,P Secreting of Yak’s Granulosa Cells Cultured in vitro

XU Geng-quan1,FAN Jiang-feng1,2,YANG Shi-hua1,LI Gu-yue1,ZHAO Hua-shan1,YU Si-jiu1,2*   

  1. (1.College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China;2.Technological Research Center of Gansu Province for Embryonic Engineering of Bovine and Sheep & Goat,Lanzhou 730070,China)
  • Received:2014-10-30 Online:2015-06-23 Published:2015-06-23

Abstract:

This experiment was conducted to study the effect of follicle stimulating hormone(FSH),luteinizing hormone(LH) on apoptosis and steroids hormone secretion of yak’s granulosa cells cultured in vitro,reveal the mechanism of gonadotropin regulating follicle development.In vitro culture system was established and the growth characteristics,apoptotic morphology were observed using cell culture and micro examination technology.The effect of FSH,LH on apoptosis and estradiol (E2),progesterone (P) secreting of yak’s granulosa cells cultured in vitro was analyzed using flow cytometry (FCM) and radioimmunoassay (RIA).The results showed that:Cultured yak’s granulosa cells demonstrated typical epithelioid growth characteristic and had “S” shape growth curve.Occasionally,the apoptotic morphological characteristics such as nuclear pyknotic and margination,endoplasmic reticulum loosen can be found in the cell culture dishes.With the increase of FSH concentration from 0.050 to 5.000 IU•mL-1,the apoptotic rate decreased and the E2,P level be on the rise.When FSH reached 5.000 IU•mL-1,the apoptotic rate down to 7.3% which was very significantly from control group (P<0.01).The apoptosis of granulosa cells were inhibited and the E2,P secreting were prompted when LH was added in a small quantity (0.050-0.500 IU•mL-1),on the other hand,high concentration of LH(5.000 IU•mL-1) prompted the apoptosis of granulosa cells and inconspicuously effected the E2,P secreting.These results indicated that FSH and LH played an important role in the regulation of follicle development and ovulation through adjust the apoptosis and steroids hormone secretion of granulosa cells in yak.

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